The Reverse Complement tool

Compute the reverse complement of a nucleotide sequence

There are two ways to obtain the reverse complement of a sample with DNA Sequence Assembler:

1. Using the 'Reverse Complement' menu
2. Using the stand-alone 'Reverse Complement' tool

Both functions support the IUPAC DNA ambiguity code.

1) The Reverse Complement menu

• Open a sample (Abi, Scf, Fasta, Seq, Txt) in Sample Viewer
• Go to Sample menu and press Reverse complement
• The RC (reverse complement) of that sample is displayed; in the case of abi or scf sample, the chromatogram is also reversed

2) The stand-alone Reverse Complement tool

• In the main DNA Baser window go to Tasks menu and click the 'Reverse Complement Tool'. The Reverse Complement Tool will open in a new window
• Enter the DNA sequence you want to reverse in the top panel and press the main button
• It's reverse complement will be displayed in the lower panel

Notes

• IUPAC ambiguity codes of the two possible nucleotides are converted as follows: R↔Y, K↔M, W and S are left unchanged.
• Ambiguity codes of the three possible nucleotides are converted as follows: D↔H, B↔V.
• The program preserves upper and lower case. This is useful for marking regions of interest.
• DNA and RNA sequences are converted into reverse-complementing sequence of DNA.
• Unknown/invalid symbols (for example '?') are left unchanged.
• This tool is available for free.