Manual end trimming

DNA Baser automatically detects and trims the untrusted regions from your samples! However, DNA Baser can automatically trim low quality ends only if your samples contain confidence score info (confidence score). If they don't contain confidence score then you can manually trim the ends:

1. Mark the region you want to cut

2. Right click to pop-up the contextual menu

3. Choose 'Mark as untrusted'

All selected bases will turn gray which means that they will be ignored (they will not be used in the contig).

Hint!

If you want to assemble multiple files, you don't have to clean each individual sequence! Instead of cleaning each sequence just cut from the contig the regions (the beginning and the end) that correspond to the low quality ends in the input samples.

Example:

In the figure below we see that both input samples have poor bases at the left end (1). Instead of cleaning each sample, we just mark the contig (2) and then we pop the contextual menu and chouse 'Delete bases'. Deleted bases are shown as gaps (-).

Index

• Automatic end trimming
• What is a chromatogram?
• What is a good chromatogram?
• FAQ about confidence scores (confidence score)
• Untrusted regions