What are chromatograms
A chromatogram (sometimes also called electropherogram) is the visual representation of a DNA sample produced by a sequencing machine (such as Applied Biosystems ABI PRISM 7700 Sequence
Fig. 1 - Example of chromatogram. The green bars above chromatogram peaks high confidence scores.
Chromatogram files can be easily converted to ABI, FASTA, SEQ or TXT with tools like DNA Baser Sequence Assembler, DNA Chromatogram Explorer, Batch ABI to FASTA converter. Please see the Download page for details.
With DNA Baser it is easy to make the difference between a good (trusted) chromatogram and a poor one. Just look at the green bars displayed above each base. These bars represents the confidence scores. A high bar means that the base call can be trusted. A low bar means that the base call cannot be trusted.
Usually, poor chromatograms enter unwanted ambiguities in your contig. However, if you assemble a good chromatogram and a poor one, DNA Baser will know how to extract the useful information from he good chromatogram and give less trust to the bad chromatogram.
- no ambiguities
- some ambiguities
- the difference between noise and signal is very small
- the overall amplitude of the signal is small
- overlapping peaks
- low confidence in bases assignment (low confidence score)
FAQ about chromatograms
Some of my ABI chromatogram files are corrupted
Why the SCF format is better than ABI format?
Details about chromatogram files
About Heracle BioSoft
Heracle BioSoft provides effective, biologist friendly, easy-to-use sequence assembly and analysis software tools to meet the ever changing needs of today’s genetic/medical researcher and diagnostician. All of our software tools are the result of close, effective collaborations with the genetic community. Therefore, if one of our software programs doesn't meet your exact needs, please do not hesitate to contact us.
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