NextGen SFF demultiplexing tool
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FastQ convertor
Convert SFF file

NCBI Database Builder

The way bioinformatics should be...


NCBI Database Builder is a tool from Avalanche NextGen biology software package that allows you to create Blast databases from your own Fasta files.


Automatically download and install NCBI Blast+ package


NCBI Database Builder tool is the equivalent of NCBI's makeblastdb command that create Blast databases. However, NCBI Database Builder offers an easy to use graphic interface and an embedded manual.



FastQ dereplicate



NCBI Database Builder





Download time

< 5 seconds

Sequence Workbench is now part of the Avalanche NextGen package. Please download this package.


  • < 3MB of disk space
  • < 30MB free RAM
  • No Java, no .Net
  • Windows XP and up
  • No installation required



This software tool is really small so you can easily copy it on a floppy disk or USB flash stick and take it with you or send it to your colleagues via email.


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Feedback/News list


This tool is Nextgen SFF/fastq analysis software. Please let us know how to make it better. We are interested in:

  • Feature requests
  • Platform you are interested in (Windows, Mac, Linux)
  • A name for the program :)


SFF to FastQ to FASTA converter