Reverse complement tool

There are two ways to obtain the reverse complement of a sample with DNA Baser:

1. using the Reverse Complement menu applied on the currently selected sample opened in Sample viewer or in Assembly grid.
2. using the Reverse Complement Tool

Both functions support the IUPAC DNA ambiguity code.

1) The Reverse Complement menu

• open a sample (abi, scf, fasta, seq, txt) in Sample viewer
• go to Chromatogram menu and press Reverse complement
• the RC sample is displayed; in the case of abi or scf files, the chromatogram is also RC

2) The Reverse Complement dedicated tool

• in the main window go to View tab and press Reverse Complement Tool
• the Reverse Complement Tool will be opened in a new window
• load from a file or paste your sequence in the upper panel and press START
• the RC sequence is displayed in the lower panel; you can either copy it to clipboard or save it to file