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Reverse complement function

Reverse complement tool

 

 

 

 

There are two ways of doing reverse complement with DNA Baser:
  a) using the Reverse Complement function applied to a sample opened in sample viewer
  b) using the Reverse Complement Tool

 

Both functions support the IUPAC DNA ambiguity code.

 

 

a) The Reverse Complement menu function:


- open a sample (abi, scf, fasta, seq, txt) in sample viewer
- go to Chromatogram menu and press Reverse complement
-
the RC sample is displayed; in the case of abi or scf files, the chromatogram is also RC

 

Reverse complement. supports abi or scf chromatogram

 

 

 

b) The Reverse Complement dedicated tool:

 

- in the main window go to View tab and press Reverse Complement Tool
-
the Reverse Complement Tool will be opened in a new window
- load from a file or paste your sequence in the upper panel and press START
-
the RC sequence is displayed in the lower panel; you can either copy it to clipboard or save it to file

 

reverse complement DNA sequence

 

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