What is a good chromatogram?

 


High quality reads:

- no ambiguities
- no noise
- peaks very well spaced

Good quality reads:

- no ambiguities
- some noise (notice baselines)
- peaks very well spaced

Medium quality regions:

- some ambiguities
- no noise
- peaks well spaced
- some homopolymeric stretches are not well resolved

Poor quality reads:

- some ambiguities
- bad noise (notice baselines)
- overlaping peaks
- low confidence in bases assignment (low QV)

Note: As you can see, this abi file contains no information about QV.

See also:

      - About quality values (QV)
      - Automatic bad ends trimming