What is a good chromatogram?

High quality reads:

- no ambiguities
- no noise
- peaks very well spaced

Good quality reads:

- no ambiguities
- some noise (notice baselines)
- peaks very well spaced

Medium quality reads:

- some ambiguities
- low noise
- peaks are still well spaced
- some homopolymeric stretches are not well resolved

Poor quality reads:

- some ambiguities
- the difference between noise and signal is very small

- the overall amplitude of the signal is small
- overlapping peaks
- low confidence in bases assignment (low QV)

Details about chromatogram files

• What is a chromatogram
• What is a good chromatogram
• FAQ about quality values (QV)
• Automatic low quality ends trimming
• Untrusted regions